Lysiander

Hi, im relativly new with photoshop and encountereda huge problem for me. I Managed to put my images into the UI, however, everytime i try to do it with an Image i've edited with Photoshop for a transparend backround, I only get the transparent Backround in-game. I thought it might be because of the different picture levels, but I put the picture in a new one so i have just *backround* in the level box and it still doesn't work. Anyone knows how I can fix that please?